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on the chromatogram vs in the chromatogram

Both 'on the chromatogram' and 'in the chromatogram' are correct, but they are used in different contexts. 'On the chromatogram' is used when referring to specific peaks, spots, or lines visible on the chromatogram, while 'in the chromatogram' is used when discussing substances or components that are present within the chromatogram.

Last updated: March 26, 2024 • 1059 views

This phrase is correct and commonly used when referring to specific peaks, spots, or lines visible on the chromatogram.

" on the chromatogram "

Use 'on the chromatogram' when describing specific peaks, spots, or lines that are visible on the chromatogram.

Examples:

  • The peak on the chromatogram corresponds to compound X.
  • There are several spots on the chromatogram indicating different substances.
  • The lines on the chromatogram represent different components of the mixture.
  • The wavelength of maximum absorption of the sample and of the standard spectra, recorded at the peak apex on the chromatogram, must be within a margin determined by the resolving power of the detection system.
  • Assume that all sterols present in the sample are represented on the chromatogram, the total area of the peaks represents 100 % of the sterol constituents and that the sterols give the same detector response.
  • the wavelength of maximum absorption of the sample and of the standard spectra, recorded at the peak apex on the chromatogram, must be the same within a margin determined by the resolving power of the detection system.
  • between 225 and 300 nm, the sample and standard spectra recorded at the peak apex on the chromatogram, must not be different for those parts of the spectrum within the range 10 % to 100 % of relative absorbance.
  • between 250 and 400 nm, the sample and standard spectra recorded at the peak apex on the chromatogram, must not be different for those parts of the spectrum within the range 10 % to 100 % of relative absorbance.
  • between 220 and 350 nm, the sample and standard spectra recorded at the peak apex on the chromatogram must not be different for those parts of the spectrum within the range 10 % to 100 % of relative absorbance.
  • between 225 and 400 nm, the sample and standard spectra recorded at the peak apex on the chromatogram, must not be different for those parts of the spectrum within the range 10 % to 100 % of relative absorbance.
  • Under the conditions described above, compounds naturally present in the wine will not produce interfering peaks on the chromatogram of the sample solution.

Alternatives:

  • visible on the chromatogram
  • observed on the chromatogram
  • present on the chromatogram
  • detected on the chromatogram
  • appearing on the chromatogram

This phrase is correct and commonly used when discussing substances or components that are present within the chromatogram.

"in the chromatogram "

Use 'in the chromatogram' when referring to substances or components that are present within the chromatogram.

Examples:

  • There are several compounds in the chromatogram.
  • The impurity was detected in the chromatogram.
  • The peaks in the chromatogram represent different analytes.
  • Total area (AT) is the sum of all the peaks appearing in the chromatogram from C14:0 to C24:0, except that corresponding to squalene.
  • The principal spots in the chromatogram are those of 1,1-GPM and 1,6-GPS.
  • The main peak in the chromatogram obtained following the procedure in Method of Assay corresponds to either stevioside or rebaudioside A
  • Comparison with an appropriate reference standard of Maltitol shows that the principle peak in the chromatogram of the test solution is similar in retention time to the principal peak in the chromatogram obtained with the reference solution (ISO 10504:1998).
  • The remaining triglycerides (peak area) in the chromatogram, including cholesterol (peak near to C24) are multiplied by the respective response factors of the standard fat (last calibration) and altogether normalized to 100.
  • The nearest peak maximum in the chromatogram shall be separated from the designated analyte peak by at least one full peak width at 10 % of the maximum height of the analyte peak.
  • If the sample contains thiamine, the thiamine peak in the chromatogram appears shortly before the amprolium peak.
  • Comparison with an appropriate reference standard of Isomalt shows that the 2 principal peaks in the chromatogram of the test solution are similar in retention time to the 2 principal peaks in the chromatogram obtained with the reference solution.

Alternatives:

  • within the chromatogram
  • contained in the chromatogram
  • found in the chromatogram
  • identified in the chromatogram
  • present in the chromatogram

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